Center of Oncocytogenomics

The Center of Oncocytogenomics (COC) workplace receives samples of tumor cells from the General University Hospital, University Hospital Motol, the Neurosurgery and Neuro-Oncology Clinic of the Central Military Hospital and other medical facilities from Prague and the surrounding area and provides super-advisory cytogenetic counseling for the entire Czech Republic. The center mainly deals with cytogenetic and molecular cytogenomic diagnostics of acquired chromosomal aberrations in tumor cells in adults and children with various types of hematological malignancies and/or with selected types of solid tumors. In addition, the department also performs cytogenetic examinations of peripheral blood lymphocytes of bone marrow donors and patients with certain congenital endocrinological syndromes associated with chromosomal abnormalities. At the workplace, all available classical and molecular cytogenomic methods are introduced and routinely used for diagnostic and research purposes: conventional cytogenetic analysis of G-banded chromosomes in metaphase, various modifications of the fluorescence in situ hybridization method (I-FISH, cIg FISH, WCP-FISH, mFISH, mBAND), microarray technologies (aCGH, aCGH/SNP), the MLPA method and massively parallel sequencing (MPS). The department has also long-term involvement in the education of undergraduate and postgraduate students and the specialized training of doctors, non-physicians and laboratory technicians (Clinical Genetics, Medical Genetics). COC workers were and are the investigators and co-investigators of a number of Czech and European research projects focused on the study of acquired genetic changes in tumor cells (e.g. IGA projects MZČR, AZV, GAČR, MŠMT, ELN, EWOG MDS, COST, etc.). The Center for Oncocytogenomics of General University Hospital cooperates very closely with the Cytogenetic Laboratory of Institute of Hematology and Blood Transfusion and, based on the agreement between the two institutions from 2002, form Center for Oncocytogenomics.