Instructions for Practical Lessons in Winter Semester

Safety Rules for Work in Student Laboratory

Practical Lesson No 1:
Introduction to work in chemical laboratory. Safety rules. Measuring volumes, centrifugation, filtration, dissolution, separation of substances from mixtures.
Instructions. Report form.

Practical Lesson No 2:
Qualitative and quantitative analysis in inorganic chemistry: Reactions of cations and anions. Titrations.
Qualitative analysis. Quantitative analysis. Instructions. Report form.

Practical Lesson No 3:
Spectrophotometry in biochemistry.
Optical methods. Instructions. Report form.

Practical Lesson No 4:
Buffers, buffer capacity. Oxidation and reduction. Electrode processes.
Titration curves. Buffers. Electrochemistry. Instructions. Report form.

Practical Lesson No 5:
Basic organic reactions. Properties of proteins. Electrophoresis.
Electrophoresis in biochemistry. Proteins - principles. Organic reactions. Instructions. Report form.

Practical Lesson No 6:
Separation techniques: Thin-layer chromatography, gel filtration, dialysis.
Separation techniques. Instructions. Report form.

Practical Lesson No 7:
Selected reactions of saccharides. Biochemical examination of diabetes mellitus.
Reactions of saccharides. Diabetes mellitus. Instructions. Report form.

Practical Lesson No 8:
Specificity of enzymes, effect of pH on enzyme activity. Redox enzymes. 
Theory and instructions. Report form.

Practical Lesson No 9:
Reactions of lipids. Digestion of lipids. Biochemical examination of lipid metabolism.
Lipids. Lipoproteins. Instructions. Report form.

Practical Lesson No 10:
Metabolism of amino acids. Nitrogen balance.
Theory. Instructions. Report form.

Practical Lesson No 11:
Immunochemistry.
Immunochemical methods. Principles. Instructions. Report form.